Immunofluorescence:Article Title: TUDCA protects against tunicamycin-induced apoptosis of dorsal root ganglion neurons by suppressing activation of ER stress
Article Snippet: .. Antibodies used in the present study A, Immunofluorescence staining Antibody Supplier Dilution Cat.no. β-tubulin Cell Signaling Technology, Inc. 1:200 2146S Anti-GFAP Abcam 1:200 ab279290 Anti-Iba1 Wako Chemicals GmbH 1:800 019-19741 Anti-Neurofilament Abcam 1:200 ab134306 Goat anti-Rabbit IgG (H+L) crossadsorbed, Alexa Fluor 488 Thermo Fisher Scientific, Inc. 1:1,000 A-11008 Goat anti-Mouse IgG (H+L) highly cross-adsorbed, Alexa Fluor 633 Thermo Fisher Scientific, Inc. 1:1,000 A-21052 B, Western blotting Antibody Supplier Dilution Cat.no. .. PERK (C33E10) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 3192 Phosphorylated-PERK (Thr980) (16F8) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 3179 eIF2α (D7D3) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 5324 Phosphorylated-eIF2α (Ser51) Cell Signaling Technology, Inc. 1:1,000 9721S IRE1α (14C10) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 3294 Anti-DDIT3 (CHOP) Abcam 1:1,000 ab11419 Anti-caspase-12 Abcam 1:1,000 ab62484 ATF6 rabbit pAb ABclonal Biotech Co., Ltd. 1:1,000 A0202 GRP78 rabbit pAb ABclonal Biotech Co., Ltd. 1:1,000 A0241 Anti-Bax Abcam 1:1,000 ab32503 HRK polyclonal Invitrogen; Thermo Fisher Scientific, Inc. 1:1,000 PA1-86773 Cleaved caspase-3 (Asp175) Cell Signaling Technology, Inc. 1:1,000 9661 Caspase-3 Cell Signaling Technology, Inc. 1:1,000 9662 PUMA Cell Signaling Technology, Inc. 1:1,000 24633 Anti -GAPDH Rabbit pAb Wuhan Servicebio Technology Co., Ltd., 1:2,000 GB11002 HRP Goat Anti-Rabbit IgG (H+L) ABclonal Biotech Co., Ltd. 1:5,000 AS014 HRP Goat Anti-Mouse IgG (H+L) ABclonal Biotech Co., Ltd. 1:5,000 AS003 PERK, protein kinase R-like ER kinase;CHOP, C/EBP homologous protein; GRP78, glucose-regulated protein 78; GFAP, glial fibrillary acidic protein; Iba1, ionized calcium binding adaptor molecule 1.
Staining:Article Title: TUDCA protects against tunicamycin-induced apoptosis of dorsal root ganglion neurons by suppressing activation of ER stress
Article Snippet: .. Antibodies used in the present study A, Immunofluorescence staining Antibody Supplier Dilution Cat.no. β-tubulin Cell Signaling Technology, Inc. 1:200 2146S Anti-GFAP Abcam 1:200 ab279290 Anti-Iba1 Wako Chemicals GmbH 1:800 019-19741 Anti-Neurofilament Abcam 1:200 ab134306 Goat anti-Rabbit IgG (H+L) crossadsorbed, Alexa Fluor 488 Thermo Fisher Scientific, Inc. 1:1,000 A-11008 Goat anti-Mouse IgG (H+L) highly cross-adsorbed, Alexa Fluor 633 Thermo Fisher Scientific, Inc. 1:1,000 A-21052 B, Western blotting Antibody Supplier Dilution Cat.no. .. PERK (C33E10) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 3192 Phosphorylated-PERK (Thr980) (16F8) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 3179 eIF2α (D7D3) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 5324 Phosphorylated-eIF2α (Ser51) Cell Signaling Technology, Inc. 1:1,000 9721S IRE1α (14C10) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 3294 Anti-DDIT3 (CHOP) Abcam 1:1,000 ab11419 Anti-caspase-12 Abcam 1:1,000 ab62484 ATF6 rabbit pAb ABclonal Biotech Co., Ltd. 1:1,000 A0202 GRP78 rabbit pAb ABclonal Biotech Co., Ltd. 1:1,000 A0241 Anti-Bax Abcam 1:1,000 ab32503 HRK polyclonal Invitrogen; Thermo Fisher Scientific, Inc. 1:1,000 PA1-86773 Cleaved caspase-3 (Asp175) Cell Signaling Technology, Inc. 1:1,000 9661 Caspase-3 Cell Signaling Technology, Inc. 1:1,000 9662 PUMA Cell Signaling Technology, Inc. 1:1,000 24633 Anti -GAPDH Rabbit pAb Wuhan Servicebio Technology Co., Ltd., 1:2,000 GB11002 HRP Goat Anti-Rabbit IgG (H+L) ABclonal Biotech Co., Ltd. 1:5,000 AS014 HRP Goat Anti-Mouse IgG (H+L) ABclonal Biotech Co., Ltd. 1:5,000 AS003 PERK, protein kinase R-like ER kinase;CHOP, C/EBP homologous protein; GRP78, glucose-regulated protein 78; GFAP, glial fibrillary acidic protein; Iba1, ionized calcium binding adaptor molecule 1.
Western Blot:Article Title: TUDCA protects against tunicamycin-induced apoptosis of dorsal root ganglion neurons by suppressing activation of ER stress
Article Snippet: .. Antibodies used in the present study A, Immunofluorescence staining Antibody Supplier Dilution Cat.no. β-tubulin Cell Signaling Technology, Inc. 1:200 2146S Anti-GFAP Abcam 1:200 ab279290 Anti-Iba1 Wako Chemicals GmbH 1:800 019-19741 Anti-Neurofilament Abcam 1:200 ab134306 Goat anti-Rabbit IgG (H+L) crossadsorbed, Alexa Fluor 488 Thermo Fisher Scientific, Inc. 1:1,000 A-11008 Goat anti-Mouse IgG (H+L) highly cross-adsorbed, Alexa Fluor 633 Thermo Fisher Scientific, Inc. 1:1,000 A-21052 B, Western blotting Antibody Supplier Dilution Cat.no. .. PERK (C33E10) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 3192 Phosphorylated-PERK (Thr980) (16F8) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 3179 eIF2α (D7D3) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 5324 Phosphorylated-eIF2α (Ser51) Cell Signaling Technology, Inc. 1:1,000 9721S IRE1α (14C10) rabbit mAb Cell Signaling Technology, Inc. 1:1,000 3294 Anti-DDIT3 (CHOP) Abcam 1:1,000 ab11419 Anti-caspase-12 Abcam 1:1,000 ab62484 ATF6 rabbit pAb ABclonal Biotech Co., Ltd. 1:1,000 A0202 GRP78 rabbit pAb ABclonal Biotech Co., Ltd. 1:1,000 A0241 Anti-Bax Abcam 1:1,000 ab32503 HRK polyclonal Invitrogen; Thermo Fisher Scientific, Inc. 1:1,000 PA1-86773 Cleaved caspase-3 (Asp175) Cell Signaling Technology, Inc. 1:1,000 9661 Caspase-3 Cell Signaling Technology, Inc. 1:1,000 9662 PUMA Cell Signaling Technology, Inc. 1:1,000 24633 Anti -GAPDH Rabbit pAb Wuhan Servicebio Technology Co., Ltd., 1:2,000 GB11002 HRP Goat Anti-Rabbit IgG (H+L) ABclonal Biotech Co., Ltd. 1:5,000 AS014 HRP Goat Anti-Mouse IgG (H+L) ABclonal Biotech Co., Ltd. 1:5,000 AS003 PERK, protein kinase R-like ER kinase;CHOP, C/EBP homologous protein; GRP78, glucose-regulated protein 78; GFAP, glial fibrillary acidic protein; Iba1, ionized calcium binding adaptor molecule 1.
Article Title: Characterization of dipyridamole as a novel ferroptosis inhibitor and its therapeutic potential in acute respiratory distress syndrome management
Article Snippet: .. Antibody Sources Applications ACCTUB Santa Cruz (Cat#Sc-23950) 1:50 for IF ACSL4 Proteintech (Cat#22401-1-AP) 1:1000 for WB β-Tubulin Cell Signaling Technology (Cat2146) 1:1000 for WB CREB1 Cell Signaling Technology (Cat#9197) 1:1000 for WB FTH1 Abclonal (Cat#A19544) 1:1000 for WB GAPDH Cell Signaling Technology (Cat#5174) 1:1000 for WB GPX4 Abcam (Cat#ab125066) 1:5000 for WB HMOX1 Abcam (Cat#ab189491) 1:1000 for WB; 1:250 for IF; 1:10000 for IHC JUN Cell Signaling Technology (Cat#9165) 1:1000 for WB MUC5AC Absin (Cat#abs126767) 1:50 for IF p-CREB1 (Ser133) Cell Signaling Technology (Cat#9198) 1:500 for WB; 1:400 for IHC; 1:25 for ChIP p-JUN (Ser73) Cell Signaling Technology (Cat#3270) 1:1000 for WB p-STAT3 (Tyr705) Cell Signaling Technology (Cat#9145) 1:1000 for WB SLC7A11 Abcam (Cat#ab175186) 1:1000 for WB SOD1 Proteintech (Cat#10269-1-AP) 1:5000 for WB STAT3 Cell Signaling Technology (Cat#9139) 1:1000 for WB Table. ..
Article Title: QSOX1 facilitates dormant esophageal cancer stem cells to evade immune elimination via PD-L1 upregulation and CD8 T cell exclusion
Article Snippet: Antibodies used for immunostaining Antibodies Corporations Catalog No. Dilution α-SMA Boster BM0002 1:200 β-Tubulin Cell Signaling Technology 86298 1:400 CD3 ZSGB Biotech ZA-0503 Ready-to-use CD34 Cell Signaling Technology 26233 1:400 CD44 Cell Signaling Technology 3570 1:400 CD45 Cell Signaling Technology 70257 1:200 CD8 ZSGB Biotech ZA-0508 Ready-to-use Cleaved Caspase-3 Cell Signaling Technology 9664 1:500 Fibronectin Gene Tech GA024504 Ready-to-use Granzyme K LifeSpan BioSciences LS-C383838 1:100 Ki67 Maixin-Bio MAB-0672 1:200 KRT14 Abcam ab119695 1:100 Laminin γ2 Abcam ab210959 1:500 Pan-cytokeratin Boster BM0030 1:200 PD-L1 Cell Signaling Technology 13684 1:200 Phospho-Stat3 Cell Signaling Technology 9145 1:200 PIGR Abcam ab275020 1:200 PLET1 Abcam ab235541 1:200 PRSS22 Abcam ab197158 1:500 QSOX1 Invitrogen PA5-38009 1:300 S100A4 Abcam ab197896 1:1000 SPRR2F Invitrogen MA5-24414 1:100 Table S3. .. Antibodies used for western blotting Antibodies Corporations Catalog No. Dilution β-Tubulin Cell Signaling Technology 86298 1:2000 Caspase-3 Cell Signaling Technology 9662 1:1000 CD44 Cell Signaling Technology 3570 1:1000 CD8α Cell Signaling Technology 98941 1:1000 Cleaved Caspase-3 Cell Signaling Technology 9664 1:1000 Cyclin B1 Cell Signaling Technology 4135 1:1000 Cyclin D3 Cell Signaling Technology 2936 1:1000 Granzyme K LifeSpan BioSciences LS-C383838 1:1000 PD-1 Cell Signaling Technology 84651 1:1000 PD-L1 Cell Signaling Technology 13684 1:1000 Phospho-Stat3 Cell Signaling Technology 9145 1:1000 QSOX1 Invitrogen PA5-38009 1:1000 Stat3 Cell Signaling Technology 12640 1:1000 Table S4. ..
other:Article Title: Dapagliflozin protects against chronic heart failure in mice by inhibiting macrophage-mediated inflammation, independent of SGLT2
Article Snippet: Antibody description Source No. and dilution SGLT1 Abclonal, USA A11976, 1:1000 SGLT2 Proteintech, USA 24654-1-AP, 1:1000 α-SMA Abcam,UK ab5694, 1:1000 Col1 Cell Signaling Technology,USA 66948, 1:1000 Col3 Abclonal, USA A0817, 1:1000 Ctgf Abcam,UK Ab209780, 1:1000 β-tubulin Cell Signaling Technology, USA 2146, 1:1000 GAPDH Cell Signaling Technology, USA 2118, 1:1000
Article Title: Dapagliflozin protects against chronic heart failure in mice by inhibiting macrophage-mediated inflammation, independent of SGLT2
Article Snippet: Antibody description Source No. and dilution SGLT1 Abclonal, USA A11976, 1:1000 SGLT2 Proteintech, USA 24654-1-AP, 1:1000 α-SMA Abcam,UK ab5694, 1:1000 Col1 Cell Signaling Technology,USA 66948, 1:1000 Col3 Abclonal, USA A0817, 1:1000 Ctgf Abcam,UK Ab209780, 1:1000 β-tubulin Cell Signaling Technology, USA 2146, 1:1000 GAPDH Cell Signaling Technology, USA 2118, 1:1000
Article Title: Nucleophosmin 1 cooperates with BRD4 to facilitate c-Myc transcription to promote prostate cancer progression
Article Snippet: NPM1 Abcam Ab10530 CAPDH Cell Signaling Technology #92310 β-Tubulin Cell Signaling Technology #5666 c-Myc Cell Signaling Technology #18583 HA-Tag Cell Signaling Technology #48823 BRD4 Abcam ab75898 Goat anti-rabbit IgG-HRP Beyotime A0208 Goat anti-mouse IgG-HRP Beyotime A0216
Proximity Ligation Assay:Article Title: PIM kinases inhibit AMPK activation and promote tumorigenicity by phosphorylating LKB1
Article Snippet: .. Protein or tag Company Product Code Dilution PIM1 Santa Cruz 12H8 1:500 PIM2 Cell Signaling Technology #4730 1:1000 PIM3 Cell Signaling Technology #4165 1:1000 PIM1 (PLA assay) Merck MABC553 1:500 FLAG-tag (PLA assay) Sigma F7425 1:500 Beta-actin Cell Signaling Technology #3700 1:5000 Phospho-AMPKα (Thr172) Cell Signaling Technology #2325 1:1000 AMPKα Cell Signaling Technology #2793 1:1000 LKB1 Cell Signaling Technology #3047 1:1000 His-tag Cell Signaling Technology #12698 1:1000 FLAG-tag Sigma F1804 1:1000 Phospho-Akt Substrate (RXXS/T) Cell Signaling Technology #9614 1:1000 β-Tubulin Cell Signaling Technology #86298 1:5000 Lamin A/C Cell Signaling Technology #4777 1:5000 Phospho-Akt (Ser473) Cell Signaling Technology #4060 1:2000 Akt Cell Signaling Technology #9272 1:2000 ..
FLAG-tag:Article Title: PIM kinases inhibit AMPK activation and promote tumorigenicity by phosphorylating LKB1
Article Snippet: .. Protein or tag Company Product Code Dilution PIM1 Santa Cruz 12H8 1:500 PIM2 Cell Signaling Technology #4730 1:1000 PIM3 Cell Signaling Technology #4165 1:1000 PIM1 (PLA assay) Merck MABC553 1:500 FLAG-tag (PLA assay) Sigma F7425 1:500 Beta-actin Cell Signaling Technology #3700 1:5000 Phospho-AMPKα (Thr172) Cell Signaling Technology #2325 1:1000 AMPKα Cell Signaling Technology #2793 1:1000 LKB1 Cell Signaling Technology #3047 1:1000 His-tag Cell Signaling Technology #12698 1:1000 FLAG-tag Sigma F1804 1:1000 Phospho-Akt Substrate (RXXS/T) Cell Signaling Technology #9614 1:1000 β-Tubulin Cell Signaling Technology #86298 1:5000 Lamin A/C Cell Signaling Technology #4777 1:5000 Phospho-Akt (Ser473) Cell Signaling Technology #4060 1:2000 Akt Cell Signaling Technology #9272 1:2000 ..
Immunohistochemistry:Article Title: Characterization of dipyridamole as a novel ferroptosis inhibitor and its therapeutic potential in acute respiratory distress syndrome management
Article Snippet: .. Antibody Sources Applications ACCTUB Santa Cruz (Cat#Sc-23950) 1:50 for IF ACSL4 Proteintech (Cat#22401-1-AP) 1:1000 for WB β-Tubulin Cell Signaling Technology (Cat2146) 1:1000 for WB CREB1 Cell Signaling Technology (Cat#9197) 1:1000 for WB FTH1 Abclonal (Cat#A19544) 1:1000 for WB GAPDH Cell Signaling Technology (Cat#5174) 1:1000 for WB GPX4 Abcam (Cat#ab125066) 1:5000 for WB HMOX1 Abcam (Cat#ab189491) 1:1000 for WB; 1:250 for IF; 1:10000 for IHC JUN Cell Signaling Technology (Cat#9165) 1:1000 for WB MUC5AC Absin (Cat#abs126767) 1:50 for IF p-CREB1 (Ser133) Cell Signaling Technology (Cat#9198) 1:500 for WB; 1:400 for IHC; 1:25 for ChIP p-JUN (Ser73) Cell Signaling Technology (Cat#3270) 1:1000 for WB p-STAT3 (Tyr705) Cell Signaling Technology (Cat#9145) 1:1000 for WB SLC7A11 Abcam (Cat#ab175186) 1:1000 for WB SOD1 Proteintech (Cat#10269-1-AP) 1:5000 for WB STAT3 Cell Signaling Technology (Cat#9139) 1:1000 for WB Table. ..
Chromatin Immunoprecipitation:Article Title: Characterization of dipyridamole as a novel ferroptosis inhibitor and its therapeutic potential in acute respiratory distress syndrome management
Article Snippet: .. Antibody Sources Applications ACCTUB Santa Cruz (Cat#Sc-23950) 1:50 for IF ACSL4 Proteintech (Cat#22401-1-AP) 1:1000 for WB β-Tubulin Cell Signaling Technology (Cat2146) 1:1000 for WB CREB1 Cell Signaling Technology (Cat#9197) 1:1000 for WB FTH1 Abclonal (Cat#A19544) 1:1000 for WB GAPDH Cell Signaling Technology (Cat#5174) 1:1000 for WB GPX4 Abcam (Cat#ab125066) 1:5000 for WB HMOX1 Abcam (Cat#ab189491) 1:1000 for WB; 1:250 for IF; 1:10000 for IHC JUN Cell Signaling Technology (Cat#9165) 1:1000 for WB MUC5AC Absin (Cat#abs126767) 1:50 for IF p-CREB1 (Ser133) Cell Signaling Technology (Cat#9198) 1:500 for WB; 1:400 for IHC; 1:25 for ChIP p-JUN (Ser73) Cell Signaling Technology (Cat#3270) 1:1000 for WB p-STAT3 (Tyr705) Cell Signaling Technology (Cat#9145) 1:1000 for WB SLC7A11 Abcam (Cat#ab175186) 1:1000 for WB SOD1 Proteintech (Cat#10269-1-AP) 1:5000 for WB STAT3 Cell Signaling Technology (Cat#9139) 1:1000 for WB Table. ..
Immunostaining:Article Title: QSOX1 facilitates dormant esophageal cancer stem cells to evade immune elimination via PD-L1 upregulation and CD8 T cell exclusion
Article Snippet: .. Antibodies used for immunostaining Antibodies Corporations Catalog No. Dilution α-SMA Boster BM0002 1:200 β-Tubulin Cell Signaling Technology 86298 1:400 CD3 ZSGB Biotech ZA-0503 Ready-to-use CD34 Cell Signaling Technology 26233 1:400 CD44 Cell Signaling Technology 3570 1:400 CD45 Cell Signaling Technology 70257 1:200 CD8 ZSGB Biotech ZA-0508 Ready-to-use Cleaved Caspase-3 Cell Signaling Technology 9664 1:500 Fibronectin Gene Tech GA024504 Ready-to-use Granzyme K LifeSpan BioSciences LS-C383838 1:100 Ki67 Maixin-Bio MAB-0672 1:200 KRT14 Abcam ab119695 1:100 Laminin γ2 Abcam ab210959 1:500 Pan-cytokeratin Boster BM0030 1:200 PD-L1 Cell Signaling Technology 13684 1:200 Phospho-Stat3 Cell Signaling Technology 9145 1:200 PIGR Abcam ab275020 1:200 PLET1 Abcam ab235541 1:200 PRSS22 Abcam ab197158 1:500 QSOX1 Invitrogen PA5-38009 1:300 S100A4 Abcam ab197896 1:1000 SPRR2F Invitrogen MA5-24414 1:100 Table S3. .. Antibodies used for western blotting Antibodies Corporations Catalog No. Dilution β-Tubulin Cell Signaling Technology 86298 1:2000 Caspase-3 Cell Signaling Technology 9662 1:1000 CD44 Cell Signaling Technology 3570 1:1000 CD8α Cell Signaling Technology 98941 1:1000 Cleaved Caspase-3 Cell Signaling Technology 9664 1:1000 Cyclin B1 Cell Signaling Technology 4135 1:1000 Cyclin D3 Cell Signaling Technology 2936 1:1000 Granzyme K LifeSpan BioSciences LS-C383838 1:1000 PD-1 Cell Signaling Technology 84651 1:1000 PD-L1 Cell Signaling Technology 13684 1:1000 Phospho-Stat3 Cell Signaling Technology 9145 1:1000 QSOX1 Invitrogen PA5-38009 1:1000 Stat3 Cell Signaling Technology 12640 1:1000 Table S4.
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